Development of a Comprehensive Undergraduate Organic Chemistry Experiment within a CDIO-PBL Framework: Synthesis and Fluorescence Sensing Applications of 3-Cyano-7-diethylaminocoumarin
Develops an organic chemistry experiment demonstrating synthesis and fluorescence sensing applications in education, highlighting innovative teaching methods.
Key Points
The research aims to create an engaging organic chemistry experiment that enhances interdisciplinary skills and practical application in detecting cyanide.
Implemented a CDIO-PBL framework for the design of the experiment.
Focused on synthesizing 3-cyano-7-diethylaminocoumarin for use as a fluorescent probe.
Utilized a choline chloride/brine catalytic system to achieve high product yield.
Applied 1H/13C NMR and HRMS for structural characterization.
Created functional sensing devices for practical cyanide detection.
Achieved an 85% yield in synthesis of the fluorescent probe.
Product demonstrated strong fluorescence and high sensitivity for cyanide (LOD < 0.05 μM).
Response time of the probe was under 100 seconds.
Student evaluations showed an average performance score of 4.4 ± 0.1, indicating proficient to expert skills.
Cognitive advancement reflected in the students’ understanding based on the SOLO taxonomy.