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March 26, 2026BioTechniquesOpen Access

Isolation and profiling of single circulating tumor cells in myeloma: a new workflow for liquid biopsies

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Authors

YSYulia ShifrinOntario GenomicsAAAsieh AlikhahWestern UniversityZHZahabiya HusainOntario Genomics

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Implication

This proof-of-principle study demonstrates a new liquid biopsy workflow that isolates and profiles CTCs in myeloma, highlighting its potential for better disease monitoring.

Key Points

  • The study aims to develop and validate a novel workflow for isolating and analyzing circulating tumor cells (CTCs) in multiple myeloma patients.
  • Developed a size-based filtration workflow for isolating CTCs from peripheral blood.
  • Conducted controlled spiking experiments with RPMI 8226 myeloma cells to establish analytical limits.
  • Performed immunophenotyping and three-dimensional telomere fluorescence in situ hybridization (FISH) on isolated CTCs.
  • Analyzed nuclear and telomeric features in CTCs compared to normal lymphocytes.
  • Achieved analytical limit of detection of approximately 1 tumor cell per 10^7 white blood cells.
  • Isolated CTCs maintained nuclear integrity, allowing detailed analysis.
  • Distinct nuclear and telomeric parameters were observed in myeloma CTCs compared to normal lymphocytes.
  • The workflow proved feasible for isolated analysis in a cohort of newly diagnosed patients.

Cite This Study

Shifrin et al. (2026) studied this question.

synapsesocial.com/papers/69c4cd25fdc3bde4489190e6https://doi.org/10.1080/07366205.2026.2645352
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