What question did this study set out to answer?

The goal is to compare different physiological thresholds obtained from muscle NIRS and heart rate variability during an incremental cycling test.

March 26, 2026Research Quarterly for Exercise and Sport

Thresholds Derived From Muscle NIRS Signal, Heart Rate Variability, and Ventilatory Parameters During an Incremental Cycling Test: An Agreement Analysis

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Why the study?

The study aimed to compare physiological thresholds obtained from muscle near-infrared spectroscopy, heart rate variability, and heart rate with ventilatory parameters during a maximal incremental cycling test.

Do muscle NIRS and HRV-derived thresholds agree with ventilatory thresholds during an incremental cycling test in trained male cyclists?

Comparison

NIRS, HRV, and HR thresholds vs ventilatory thresholds (VT1 and VT2)

Design

Cross-sectional agreement analysis

Authors

ENEduardo Marcel Fernandes NascimentoUniversidade Federal de Santa Catarina FBFernando Klitzke BorszczUniversidade do Estado de Santa Catarina TVThiago Pereira VenturaUniversidade Federal de Santa Catarina

Discussion

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Implication

NIRS/HRV thresholds should complement rather than replace ventilatory ones for exercise prescription; leaves open interchangeable use pending individualized validation.

Key Points

The goal is to compare different physiological thresholds obtained from muscle NIRS and heart rate variability during an incremental cycling test.
Twenty trained male cyclists completed an incremental cycling test with breath-by-breath gas analysis.
Muscle NIRS signals, heart rate variability (HRV), and heart rates were recorded continuously.
Ventilatory thresholds (VT1 and VT2) were identified and compared to derived thresholds from muscle NIRS and HRV.
No significant differences in power-output between VT1 and metrics from muscle NIRS and HRV.
Standard error of estimate for comparisons was approximately equivalent to one incremental stage (25 W).
Agreement analysis revealed substantial inter-individual variability, limiting precision for individual prescriptions.

Structured PICO

Do muscle NIRS and HRV-derived thresholds agree with ventilatory thresholds during an incremental cycling test in trained male cyclists?

Population

Trained male cyclists

Intervention

Thresholds derived from muscle near-infrared spectroscopy (NIRS) and heart rate variability (HRV) during an incremental cycling test

Comparator

Ventilatory thresholds (VT1 and VT2)

Outcome

Agreement between ventilatory thresholds and thresholds derived from NIRS and HRVsurrogate

While mean values of NIRS and HRV-derived thresholds do not differ significantly from ventilatory thresholds, substantial inter-individual variability suggests they should complement rather than replace ventilatory thresholds for exercise prescription.

Limitations

Substantial inter-individual error limits precision for individual prescription

Cite This Study

Nascimento et al. (2026) studied this question.

synapsesocial.com/papers/69c4cd30fdc3bde448919322 https://doi.org/10.1080/02701367.2026.2645354

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Implication

NIRS/HRV thresholds should complement rather than replace ventilatory ones for exercise prescription; leaves open interchangeable use pending individualized validation.

Thresholds Derived From Muscle NIRS Signal, Heart Rate Variability, and Ventilatory Parameters During an Incremental Cycling Test: An Agreement Analysis

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Thresholds Derived From Muscle NIRS Signal, Heart Rate Variability, and Ventilatory Parameters During an Incremental Cycling Test: An Agreement Analysis

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider