Demonstrates rapid detection of UTI biomarkers, enabling timely precision nursing interventions.
This study establishes a rapid diagnostic platform using UPLC-MS/MS to quantify urinary tract infection biomarkers within 8 minutes. Targeting phenylacetylglycine (PAG, E. coli) , cyclic diguanylate monophosphate (c-di-GMP, K. pneumoniae ), and pyocyanin (PYO, P. aeruginosa ), we optimized a 3-min sample preparation and 5-min chromatographic separation. The portable Waters Xevo TQ-S micro system achieved detection limits of 0.008–0.03 μmol/L and precision <5.0% RSD using deuterated internal standard d5-PAG. Clinical validation with 326 specimens demonstrated >93% concordance with culture and 42% higher mixed-infection detection (71 vs 50), enabling pathogen-directed therapy within 45 min of admission.The rapid diagnosis facilitated precision nursing interventions including targeted catheter care and infection control measures.
No takes yet. Share an insight, caveat, or question.
He et al. (2026) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: