Wcn26-2630 Urinary Follistatin-Like 3 Is a Marker Reflecting the Severity of Tubular Damage in Patients With Acute Kidney Injury

underwent splenectomy or total body irradiation (TBI) for LSK cell depletion before CpG-ODN treatment and CLP.Results: CpG-ODN pretreatment induced significant splenomegaly (mean 191mg in CpG-ODN group vs. 71mg in vehicle group, P < 0.0001) and increased splenic LSK cell counts (mean 1,052,854 vs. 56,683 cells, P < 0.0001).These effects were not observed in TLR9 KO or IL-17 KO mice, confirming dependence on TLR9-and IL-17dependent pathways.CpG-ODN pretreatment significantly improved survival after CLP and reduced BUN levels (39.6 vs. 62.7 mg/dL, P = 0.00065).In contrast, no survival benefit of CpG-ODN pretreatment was observed in TLR9 KO or IL-17 KO mice.Importantly, splenectomy or TBI for LSK cell depletion abolished the survival advantage conferred by CpG-ODN, suggesting that the mobilization of LSK cells in the spleen is essential for protection.Conclusion: Pretreatment with CpG-ODN improves sepsis survival and preserves kidney function, potentially through TLR9-IL-17-dependent immune pathways and mobilization of splenic LSK cells.These findings identify a novel immunomodulatory pathway that may inform therapeutic strategies to improve sepsis outcomes.I have no potential conflict of interest to disclose.I did not use generative AI and AI-assisted technologies in the writing process.