Multidimensional techniques enhance compound separation capacity in liquid chromatography, suggesting improved efficiency.
The quest for a substantial increase in the peak capacity and, therefore, in the number of compounds that can be separated in a single chromatographic run, led to the development of multidimensional techniques in high-performance liquid chromatography (HPLC). The “classical” method of collecting fractions from the initial separation and separating them further in the secondary steps is very powerful but its fully automated “online” version requires quite complex instrumentation. In contrast, development of new chromatographic media with segregated bimodal chemistries allows the 2-D separations to be achieved in a single column with standard HPLC equipment.
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František Švec (1997) studied this question.
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