From dissolution to dermal benefits: In vitro, ex vivo , and in vivo evaluation of mesoporosil ® as a highly bioavailable silicium source

Objectives: The objective of this study is to assess how effectively Mesoporosil ® , a next-generation mesoporous silica ingredient, dissolves, is absorbed in the intestine, and becomes bioavailable in the body, with the aim of improving silicium delivery for skin and connective tissue health. Material and Methods: A comprehensive assessment was performed using in vitro, ex vivo , and in vivo models. Dissolution kinetics of Mesoporosil ® were quantified in water and fasted state simulated intestinal fluid (FaSSIF) using inductively coupled plasma optical emission spectrometry and silicomolybdic acid spectrophotometry. Ex vivo intestinal transport was evaluated using Ussing chambers on isolated rat jejunum to determine the apparent permeability coefficient (Papp) and the corresponding absorbed fraction (AF). Pharmacokinetic profiling was conducted in Sprague–Dawley rats after oral administration of Mesoporosil ® microcapsules, measuring key parameters including Cmax, Tmax, and area under the curve (AUC). Results: Mesoporosil ® exhibited rapid and extensive silicium release, achieving approximately 90% dissolution after 8 h in water, about 380-fold higher than standard mesoporous silica and up to 27-fold greater silicium liberation in FaSSIF compared with commercial silicium supplements. Ex vivo permeability testing showed a Papp of 3.35 × 10 −5 cm / s, corresponding to an estimated AF of 96.7%, indicating highly efficient intestinal transfer. In the in vivo model, Mesoporosil ® demonstrated fast systemic uptake with a Cmax of approximately 73 ng/mL, a Tmax of 1 h, and an AUC of roughly 274 ng•h/mL. These data confirm effective oral absorption and robust bioavailability of silicium delivered through Mesoporosil ® . Conclusion: Across all experimental models, Mesoporosil ® showed enhanced dissolution, absorption, and bioavailability compared with conventional silicium sources. These benefits are attributed to its optimized mesoporous structure, which promotes efficient hydrolytic conversion to orthosilicic acid, the bioactive and absorbable form of silicium. The demonstrated performance supports Mesoporosil ® as a highly bioavailable silicium ingredient with strong potential to benefit skin and connective tissue health through improved silicium delivery.