Two demonstrations illustrate how bandpass influences calibration curves for measuring chlorine and quinine.
Two experiments are presented. The first is a breadboard exercise in which students assemble all or part of a filter photometer that can quantify chlorine in drinking water. Bandpass interference filters can be easily interchanged to demonstrate the effect of bandpass on the slope and the linearity of a calibration curve when measuring the absorbance of electromagnetic radiation. In the second exercise, components of the instrument are rearranged to make a filter fluorometer that quantifies quinine in tonic water. Exchanging filters in the fluorometer varies the intensity of electromagnetic radiation incident on the sample and the intensity of the emitted electromagnetic radiation that reaches the detector. The effects of these changes on the slope of a calibration curve in a fluorescence experiment are then demonstrated.
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Tommey et al. (2014) studied this question.
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