This project develops a system to teach column chromatography techniques in educational settings, improving student engagement.
The aim of this project is to develop a simple system that can be used to demonstrate the concepts and techniques of column chromatography to students from high school to university level. The system uses commonly available materials and apparatus, and complete experiments can be carried out within the timeframe of school laboratory-based lessons. A simple, cheap and reliable way to synthesize agarose microbeads for use as the stationary phase in a column is described. Mixture of dyes are also identified and optimized so that they separate cleanly into distinct bands in the column, and are therefore suitable for demonstration and student-led experimentation purposes.
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Lee et al. (2011) studied this question.
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