Laboratory experiment demonstrates photodynamic therapy's effectiveness via porphyrin in carcinoma cells, suggesting a potent treatment method.
A convenient procedure to visualize the photodynamic effect of porphyrins on cell lines is shown. 5,10,15,20-Tetra(4- methoxyphenyl)porphyrin (TMP) was used as photosensitizer. The culture flasks bearing a Hep-2 cell line were incubated for 24 h with 1 mM of TMP. Under these conditions saturation of the TMP intracellular concentration is obtained. The irradiation of cell cultures for 30 min produces 90% cell mortality, while no toxicity was observed under dark conditions or under irradiation without TMP. This methodology can be used to demonstrate the photodynamic therapy (PDT) process in a laboratory experiment.
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Alvarez et al. (2000) studied this question.
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