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March 29, 2026Прикладная биохимия и микробиология / Applied Biochemistry and Microbiology

Competitive Polarization Fluorescence Optamer Analysis of Aflatoxin B1: Influence of Salt Composition of the Reaction Medium on the Interaction of Reagents and Detection Limit

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Authors

ASA.V. SamokhvalovAZA.V. ZherdevA N Bach Institute of BiochemistryBDB.B. DzantievA N Bach Institute of Biochemistry

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Implication

Research demonstrates binding interactions of aflatoxin B1 with aptamers, indicating the role of cation composition.

Key Points

  • The aim is to assess how different cations in the reaction medium impact the binding of aflatoxin B1 to an aptamer.
  • Studied interaction between fluorescein-labeled aflatoxin B1 and an aptamer.
  • Analyzed effect of mono- and divalent cations at varying concentrations.
  • Measured fluorescence anisotropy to assess binding reactivity.
  • FA changes were significant and dependent on the specific cation and its concentration.
  • Monovalent cations affected FA at concentrations of 400 mM–2.5 M.
  • Divalent cations showed stronger effects within the range of 6 to 200 mM.

Cite This Study

Samokhvalov et al. (2025) studied this question.

synapsesocial.com/papers/69c8c2b8de0f0f753b39d1f3https://doi.org/10.7868/s3034574x25050092
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Also Consider

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  1. 1Polycations as Aptamer-Binding Modulators for Sensitive Fluorescence Anisotropy Assay of Aflatoxin B12024 · 5 citations
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  3. 3Rigidity-Responsive Fluorescence Polarization Detection of Aflatoxin B 1 via Programmable RCA-Coupled CRISPR/Cas12a and a Conformation-Restricted Depolarization Reporter2026 · 3 citations
  4. 4An Electrochemical Aptasensor with Enhanced Electrocatalytic Signal by Electrode Modification Material for Aflatoxin B1 Detection-Based Bipedal DNAzyme Cleavage Effect2025
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