Successful Endoscope-Assisted Transcervical Insemination (TCI) in Dogs Using Sperm Recovered from Epididymides Stored at 5 °C for 24 h After Castration Prior to Semen Collection and Cryopreservation
Evaluates endoscopic insemination success using cryopreserved sperm in dogs, suggesting a non-surgical alternative.
Key Points
The aim is to determine if sperm from refrigerated epididymides retains fertilizing ability after cryopreservation for use in non-surgical insemination.
Stored epididymides at 4–5 °C for 24 h after castration
Recovered spermatozoa via single-incision aspiration
Frozen using the Uppsala method and stored in liquid nitrogen
Inseminated a female dog in heat with thawed sperm through transcervical endoscope guidance
Confirmed pregnancy through ultrasonography
Delivered nine healthy puppies at term
Achieved insemination with 62 × 10^6 progressive motile spermatozoa
Demonstrated that 24 h refrigeration does not impair fertilizing ability of sperm