italicShoutai Pill /italic（寿胎丸） attenuates maternal-fetal interface inflammation by inhibiting NLRP3-mediated trophoblast pyroptosis

Key Points

• The research aims to explore how Shoutai Pill affects recurrent miscarriage and its role in regulating trophoblast pyroptosis through NLRP3.
• Utilized a recurrent miscarriage mouse model using CBA/J×DBA/2 mice
• Divided into treatment groups: model, progesterone, low-dose and high-dose Shoutai Pill
• Administered treatment for 14 days
• Monitored embryo resorption rates and performed histological analyses
• Conducted ELISA and Western blot assessments of inflammatory markers and NLRP3 activation
• The model group showed significantly increased embryo resorption rates compared to controls (<i>P</i>&lt;0.01)
• Elevated serum inflammatory factors IL-1β and IL-18 were noted in the model group (<i>P</i>&lt;0.01)
• NLRP3 and related proteins showed higher expression levels in model group placenta (<i>P</i>&lt;0.01)
• High-dose Shoutai Pill and progesterone groups had decreased embryo resorption rates (<i>P</i>&lt;0.01)
• Inflammatory markers and NLRP3 co-expression in trophoblasts significantly reduced in treatment groups (<i>P</i>&lt;0.01)