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The aim is to evaluate the effectiveness of a small helper Ti-plasmid in enhancing T-DNA transfer from Agrobacterium to plant cells.

Small Helper Ti-plasmid Coexisting With the A281virF Gene Encoding an F-Box–Like Protein Improves the Efficiency of T-DNA Transfer From Agrobacterium Cells to Plant Cells

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The aim is to evaluate the effectiveness of a small helper Ti-plasmid in enhancing T-DNA transfer from Agrobacterium to plant cells.
Constructed a small helper Ti-plasmid (pCU307D) from pTiEHA101.
Examined T-DNA transfer efficiencies using Arabidopsis cultured cells.
Compared GUS activities between strains carrying pCU307D and conventional strains.
T-DNA transfer efficiency was similar for EHA101 and C58C1 with pCU307D.
pCU307D had 2.8-fold higher copy numbers than EHA101.
Co-culture with CU307DF resulted in 3.2-fold higher GUS activities than EHA101.

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Researchers demonstrate improved T-DNA transfer efficiency using a modified Agrobacterium strain in Arabidopsis.

Abello et al. (2026) studied this question.

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