Generation and characterization of induced pluripotent stem cell lines from one Parkinson’s disease patient carrying the GBA1 T369M variant and two variant-free controls

Key Points

• To generate and characterize induced pluripotent stem cell lines from a Parkinson's disease patient with the GBA1 T369M variant and controls.
• Generated induced pluripotent stem cells from PBMCs of one PD patient and two controls.
• Characterized stem cell lines for morphology, pluripotency marker expression, and karyotype analysis.
• Ensured all lines were transgene-free and capable of differentiating into three germ layers.
• All induced pluripotent stem cell lines displayed typical morphology and expressed pluripotency markers.
• The lines maintained a normal karyotype, indicating genetic stability.
• These cell lines can be utilized to explore mechanisms related to the GBA1 T369M variant in Parkinson's disease.