Droplet microfluidics has been widely used in biological, chemical, and medical research owing to its advantages of miniaturization, high throughput, and low reagent consumption. However, limited sensitivity and optical path length in on-chip absorbance detection remain major challenges for droplet-based microfluidic analysis. Traditional absorbance detection suffers from low sensitivity due to the extremely short optical path in microfluidic channels, while existing optical path extension methods have drawbacks such as complex fabrication, easy droplet rupture, or strict incident angle requirements. To address these issues, this study developed a droplet microfluidic absorbance detection platform integrating optical fibers, on-chip micromirrors, external fluidic actuation, and an absorbance detection module. Microchannel sidewalls filled with low-melting-point metal act as mirrors; the multi-reflection optical path, combined with optical fibers and micromirrors, compensates for insufficient light manipulation and effectively extends the absorption path length, improving sensitivity and accuracy. Using this method, the detection limit for methylene blue solution was 20 μM, and the sensitivity for Escherichia coli (E. coli) suspension was doubled compared with traditional Nanodrop OD600 measurement. This device features low fabrication difficulty and cost and stable detection, providing a proof-of-concept strategy for enhanced absorbance detection in droplet microfluidic systems.
Liu et al. (Thu,) studied this question.