Sulfur assimilation determines S-adenosyl-l-methionine flux for enhancing methylation efficiency in heterologous biosynthesis

Sulfur assimilation determines S-adenosyl-L-methionine flux for enhancing methylation efficiency in heterologous biosynthesisThis study reveals that sulfur assimilation-specifically, the first reduction step of sulfate-has a determined impact on Sadenosyl-L-methionine flux compared with the C4 skeleton (activated form of L-homoserine).These findings offer a broadly applicable engineering strategy for the high-efficiency synthesis of diverse methyl-containing natural products with extensive practical applications.