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The aim is to optimise the start-up parameters of dark fermentation for maximising biohydrogen production from food waste.

Multivariate interaction analysis and optimisation of start-up parameters for maximising biohydrogen production via continuous dark fermentation of food waste

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The aim is to optimise the start-up parameters of dark fermentation for maximising biohydrogen production from food waste.
Developed and validated a model simulation for dark fermentation.
Conducted sensitivity analysis and response surface methodology optimisation.
Considered interactions among start-up hydraulic retention time, substrate concentration, temperature, and pH.
Synergistic optimisation significantly maximised biohydrogen yield.
Significant interactions were found between temperature and hydraulic retention time, and substrate concentration.
Optimal conditions were identified as 54 °C, 15 days retention time, near-neutral pH, and 42 gVS/L substrate concentration.

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Multivariate interaction analysis maximises biohydrogen yield in food waste fermentation, indicating sustainable scaling potential.

Gbiete et al. (2026) studied this question.

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