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April 5, 2026Cancer Research

Abstract 3399: Highly multiplexed imaging allows for spatial characterization of tumor organoid models

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Authors

AVAnton VillamejorJLJoseph LownikDMDaria Mandel

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Overview

Highly multiplexed imaging characterizes tumor organoid models, suggesting improved evaluation techniques for pre-clinical studies.

Key Points

  • To evaluate the effectiveness of highly multiplexed imaging in characterizing tumor organoid models and their structural representation.
  • Produced patient-derived lymphoma and glioblastoma organoids.
  • Fixed and embedded organoids in hydroxyethyl agarose gel and paraffin.
  • Utilized automated sequential immunofluorescence imaging for detecting 80 proteins with a multiplex panel.
  • Selected 50 markers for organoid profiling targeting cell phenotypes and metabolic states.
  • Lymphoma organoid showed cell type frequencies of T-cell subsets and B-cells matching flow cytometric analysis.
  • GBM organoid contained various neural cell states akin to single-plex assays.
  • Proliferative T-cells in lymphoma organoid exhibited higher glycolytic and mitochondrial enzyme expression compared to non-proliferative subsets.

Cite This Study

Villamejor et al. (2026) studied this question.

synapsesocial.com/papers/69d1fcd4a79560c99a0a27bfhttps://doi.org/10.1158/1538-7445.am2026-3399
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