Demonstrates that short-chain fatty acids suppress invasion and clonogenic survival in triple-negative breast cancer cells, suggesting potential therapeutic applications.
Key Points
The aim was to investigate the effects of short-chain fatty acids on invasion and clonogenic potential in triple-negative breast cancer cells.
MDA-MB-231 cells treated with SCFAs (butyrate, acetate, propionate) at 5 mM
Invasion assessed using Boyden chambers with Matrigel
Clonogenic assays conducted using butyrate-treated cells at densities of 1,000 cells per well for 9-10 days
Quantification of invasion and colony formation done using ImageJ.
Cells were stained for visualization of colonies and invasion.
Butyrate and propionate significantly suppressed invasion compared to control (p<0.0001 and p=0.0031)
Acetate showed no significant effect on invasion (p=0.4084)
Colony formation was completely inhibited in butyrate-treated cells at 1 mM and 5 mM
Control cells formed an average of 429 colonies per well
Treated cells exhibited loss of adherence and rounded morphology, indicating cytotoxicity.
Cite This Study
Prats-Marrero et al. (2026) studied this question.