What question did this study set out to answer?

To determine whether functional mineralocorticoid receptors and 11betaHSD2 are expressed in human vascular smooth muscle cells and mediate gene transcription in response to aldosterone and angiotensin II.

February 17, 2005Circulation ResearchOpen Access

Angiotensin II and Aldosterone Regulate Gene Transcription Via Functional Mineralocortocoid Receptors in Human Coronary Artery Smooth Muscle Cells

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Authors

IJIris Z. JaffeVascular / Pulmonary Vascular MMMichael E. MendelsohnBoston University

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Implication

In vitro study reveals mineralocorticoid receptor gene regulation by angiotensin II in human coronary smooth muscle cells, suggesting a direct vascular pathway in heart disease.

Key Points

To determine whether functional mineralocorticoid receptors and 11betaHSD2 are expressed in human vascular smooth muscle cells and mediate gene transcription in response to aldosterone and angiotensin II.
Assessed mRNA and protein expression of mineralocorticoid receptors (MR) and 11beta-hydroxysteroid dehydrogenase-2 (11betaHSD2) in cultured human coronary and aortic vascular smooth muscle cells (VSMCs).
Measured MR-mediated gene transcription following stimulation with aldosterone, cortisol, or angiotensin II, with and without spironolactone, losartan, or an aldosterone synthase inhibitor.
Profiled downstream gene activation using microarray and quantitative RT-PCR assays.
Human coronary and aortic VSMCs express functional MR and 11betaHSD2, with 11betaHSD2 inhibition enhancing cortisol-mediated gene transactivation.
Angiotensin II stimulates MR-dependent transcription in coronary VSMCs; this action is prevented by losartan and spironolactone, but unaffected by aldosterone synthase inhibition.
Aldosterone exposure upregulates endogenous gene expression programs linked to vascular fibrosis, inflammation, and calcification.

Cite This Study

Jaffe et al. (2005) studied this question.

synapsesocial.com/papers/69d5708275589c71d767dcbc https://doi.org/10.1161/01.res.0000159937.05502.d1

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Implication

In vitro study reveals mineralocorticoid receptor gene regulation by angiotensin II in human coronary smooth muscle cells, suggesting a direct vascular pathway in heart disease.

Angiotensin II and Aldosterone Regulate Gene Transcription Via Functional Mineralocortocoid Receptors in Human Coronary Artery Smooth Muscle Cells

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Implication

Key Points

Cite This Study

Angiotensin II and Aldosterone Regulate Gene Transcription Via Functional Mineralocortocoid Receptors in Human Coronary Artery Smooth Muscle Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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