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January 13, 2003The Journal of Cell BiologyOpen Access

Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles

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Population

Preclinical models including yeast two-hybrid system, in vitro pull-down assays, heterologous cells, and…

Design

Preclinical

Authors

PBPaola BagnatoUniversity of GenoaVBVirginia BaroneUniversity of SienaEGEmiliana GiacomelloUniversity of Trieste

Discussion

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Overview

Animal data extend SR-myofibril tethering models; hypothesis-generating and requires mammalian/human validation before any clinical consideration.

Structured PICO

P
Population
Preclinical models including yeast two-hybrid system, in vitro pull-down assays, heterologous cells, and differentiated skeletal muscle cells
O
Outcome
Characterization of binding sites and critical amino acids required in the interaction between ank1.5 and obscurin, and their localization in skeletal muscle cellssurrogate

The interaction between ank1.5 and obscurin provides the first direct evidence of a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles.

Cite This Study

Bagnato et al. (2003) studied this question.

synapsesocial.com/papers/69d573f8cb7e4bf698cf4050https://doi.org/10.1083/jcb.200208109
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly2001 · 309 citations
  2. 2The cytoskeleton in skeletal, cardiac and smooth muscle cells.1998 · 76 citations
  3. 3A vinculin-containing cortical lattice in skeletal muscle: transverse lattice elements ("costameres") mark sites of attachment between myofibrils and sarcolemma.1983 · 440 citations
  4. 4Preparation and morphology of sarcoplasmic reticulum terminal cisternae from rabbit skeletal muscle.1984 · 538 citations
  5. 5The ryanodine receptor/calcium channel genes are widely and differentially expressed in murine brain and peripheral tissues.1995 · 568 citations