What question did this study set out to answer?

To determine the mechanism by which low concentrations of taxol induce mitotic arrest and inhibit human cancer cell proliferation.

October 15, 1993Proceedings of the National Academy of SciencesOpen Access

Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations.

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Authors

MJMary Ann JordanUniversity of California, Santa Barbara RTRobert TosoAmgen (United States)DTDouglas ThrowerUniversity of North Carolina at Chapel Hill

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Implication

In vitro study demonstrates that low-dose taxol halts mitosis in HeLa cells by kinetically stabilizing spindle microtubules, indicating an antiproliferative mechanism shared with vinblastine.

Key Points

To determine the mechanism by which low concentrations of taxol induce mitotic arrest and inhibit human cancer cell proliferation.
Treated HeLa cells with varying concentrations of taxol to quantify cell proliferation, mitotic arrest at the metaphase/anaphase boundary, and microtubule polymer mass.
Assessed growing and shortening dynamics in vitro using microtubules reassembled from bovine brain tubulin.
Quantified the intracellular accumulation and concentration factor of taxol in cultured HeLa cells.
Taxol produced half-maximal inhibition of cell proliferation at 8 nM and half-maximal mitotic block at 8 nM without increasing microtubule polymer mass below 10 nM.
Microtubule polymer mass increased half-maximally at 80 nM and reached a maximum of 5 times normal levels at 330 nM taxol.
Taxol concentrated several hundredfold in HeLa cells to levels that suppressed microtubule dynamic instability in vitro, kinetically stabilizing spindle microtubules.

Cite This Study

Jordan et al. (1993) studied this question.

synapsesocial.com/papers/69d6b9bd8dca315383ed8abc https://doi.org/10.1073/pnas.90.20.9552

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Implication

In vitro study demonstrates that low-dose taxol halts mitosis in HeLa cells by kinetically stabilizing spindle microtubules, indicating an antiproliferative mechanism shared with vinblastine.

Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations.

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Implication

Key Points

Cite This Study

Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations.

Discussion

Member takes

Implication

Key Points

Cite This Study

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