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The aim is to develop a reporter mouse strain for evaluating the specificity of Cre recombinase expression.

March 27, 2000FEBS LettersOpen Access

A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre‐mediated recombination

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The aim is to develop a reporter mouse strain for evaluating the specificity of Cre recombinase expression.
Developed a transgenic mouse strain expressing enhanced green fluorescent protein (EGFP) upon Cre-mediated recombination.
Conducted a cross between the reporter strain and a transgenic strain with Cre expression in zygotes.
Monitored EGFP fluorescence to evaluate Cre expression directed by various promoters.
Progeny from the cross exhibited ubiquitous EGFP fluorescence, demonstrating successful Cre-mediated recombination.
The developed reporter strain allows monitoring of Cre expression during embryogenesis under regulated promoters.

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Transgenic mice express enhanced green fluorescent protein via Cre-mediated recombination, highlighting Cre specificity.

Kawamoto et al. (2000) studied this question.

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