What question did this study set out to answer?

To establish and validate a high-throughput amplicon sequencing protocol on Illumina HiSeq2000 and MiSeq platforms and verify whether biological conclusions are consistent between instruments.

March 8, 2012The ISME JournalOpen Access

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

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Authors

JCJ. Gregory CaporasoRutgers, The State University of New Jersey CLChristian L. LauberNational Institutes of Health WWWilliam A. WaltersMax Planck Institute for Biology

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Implication

Validation study demonstrates consistent microbial community profiling across sequencing platforms, indicating reliable high-throughput amplicon analysis.

Key Points

To establish and validate a high-throughput amplicon sequencing protocol on Illumina HiSeq2000 and MiSeq platforms and verify whether biological conclusions are consistent between instruments.
Developed an amplicon sequencing protocol configured for the Illumina HiSeq2000 and MiSeq platforms.
Sequenced 24 microbial communities representing both host-associated and free-living environments across distinct amplicon target regions.
Successfully recaptured known biological community compositions across all 24 evaluated environmental and host-associated samples.
Yielded consistent biological conclusions across instruments (HiSeq2000 versus MiSeq) and across different sequenced amplicon regions without platform-driven divergence.

Cite This Study

Caporaso et al. (2012) studied this question.

synapsesocial.com/papers/69d6c3e8abefa4d4d4aa8078 https://doi.org/10.1038/ismej.2012.8

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Implication

Validation study demonstrates consistent microbial community profiling across sequencing platforms, indicating reliable high-throughput amplicon analysis.

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

Discussion

Member takes

Implication

Key Points

Cite This Study

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

Discussion

Member takes

Implication

Key Points

Cite This Study

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