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Most mitochondria prepared by differential centrifugation are contaminated to some extent by lysosomes, peroxisomes, tubular Golgi membranes, and small amounts of endoplasmic reticulum. Density gradient centrifugation using a variety of density media--sucrose, Percoll, Nycodenz, Iodixanol--is described here and is used to prepare purer fractions of mitochondria. The resulting gradient can be analyzed for three marker enzymes: succinate dehydrogenase (mitochondria), b-galactosidase (lysosomes), and catalase (peroxisomes).
John M. Graham (Fri,) studied this question.