What question did this study set out to answer?

Investigate the specific cellular immune mechanisms and the role of T lymphocytes in mediating cardiac inflammation during myosin-induced acute myocarditis.

October 1, 1991The Journal of ImmunologyOpen Access

Myosin-induced acute myocarditis is a T cell-mediated disease

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Why the study?

Is myosin-induced acute myocarditis mediated by T cells, specifically CD4+ T cells, in mouse models?

Population

Mouse models induced with purified mouse cardiac myosin to cause acute myocarditis

Comparison

Adoptive transfer of purified T cells, CD4+ T… vs Transfer of serum with high-titer antimyosin…

Design

Preclinical

Authors

SSStacy C. SmithWashington University in St. Louis PAPaul M. AllenUniversity of Huddersfield

Discussion

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Implication

Supports T cell-targeted research in experimental myocarditis; leaves open translation to human disease and therapy.

Key Points

Investigate the specific cellular immune mechanisms and the role of T lymphocytes in mediating cardiac inflammation during myosin-induced acute myocarditis.
Induced acute myocarditis in mice using purified mouse cardiac myosin, followed by adoptive transfer of purified T cells or antimyosin serum into SCID recipient mice.
Depleted CD4+ or CD8+ T cells in A/J mice using monoclonal antibodies and administered MHC class II competitor peptides to test disease prevention.
Adoptive transfer of purified T cells successfully transferred myocarditis to SCID recipients, whereas transfer of high-titer antimyosin serum failed to induce disease.
Depletion of CD4+ T cells fully protected mice against myocarditis induction, while CD8+ T cell depletion only reduced inflammatory severity.
Treatment with MHC class II competitor peptides prevented disease induction, confirming that the condition is an I-Ak-restricted, CD4+ T cell-dependent disease.

Structured PICO

Is myosin-induced acute myocarditis mediated by T cells, specifically CD4+ T cells, in mouse models?

Population

Mouse models (C.B-17, SCID, A/J, and H-2k mice) induced with purified mouse cardiac myosin to cause acute myocarditis

Intervention

Adoptive transfer of purified T cells, CD4+ T cell depletion via mAb, CD8+ T cell depletion via mAb, and MHC class II competitor peptides

Comparator

Transfer of serum with high-titer antimyosin antibodies; non-depleted controls

Outcome

Induction of acute myocarditis

This preclinical study provides direct evidence that myosin-induced acute myocarditis is a T cell-mediated disease, specifically dependent on CD4+ T cells.

Cite This Study

Smith et al. (1991) studied this question.

synapsesocial.com/papers/69d71b27faf9bc6d3dbef030 https://doi.org/10.4049/jimmunol.147.7.2141

Topics

Myocarditis

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Myosin-induced acute myocarditis is a T cell-mediated disease

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Myosin-induced acute myocarditis is a T cell-mediated disease

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Topics

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