What question did this study set out to answer?

To develop and evaluate a standardized modification of the Ashcroft scale to reduce variability and improve reproducibility when quantifying lung fibrosis in small animals.

April 1, 2008SHILAP Revista de lepidopterologíaOpen Access

Standardized Quantification of Pulmonary Fibrosis in Histological Samples

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To develop and evaluate a standardized modification of the Ashcroft scale to reduce variability and improve reproducibility when quantifying lung fibrosis in small animals.
Induced lung fibrosis in Lewis rats via a single intratracheal dose of 0.3 mg/kg bleomycin (n = 13) compared with saline controls (n = 4).
Modified the Ashcroft scale by creating explicit, standardized criteria for assigning fibrosis severity grades from 0 to 8 on histological sections.
Randomly assigned 32 observers to score 108 slide photographs using either the original Ashcroft scale or the modified scale, and correlated scores with in vivo lung computed tomography (CT) scans.
The modified scale significantly lowered score standard deviation variability compared to the original Ashcroft scale (mean variability 0.25 vs 0.62, P < 0.0001).
Inter-observer agreement improved substantially with the modified scale (kappa 0.65–0.93 vs 0.23–0.59, P < 0.0001), as did intra-observer agreement (kappa 0.87–0.91 vs 0.51–0.74, P < 0.05).
Histological evaluations using the modified scale showed a stronger correlation with in vivo lung CT scans than the original Ashcroft scale (R = 0.58 vs R = 0.33).

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Implication

Randomized observer study demonstrates improved reproducibility in quantifying rodent pulmonary fibrosis with a modified Ashcroft scale, indicating a more reliable standard for histological...

Cite This Study

Hübner et al. (2008) studied this question.

synapsesocial.com/papers/69d74c8fb1cb92dd1bb8a58a https://doi.org/10.2144/000112729

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Implication

Randomized observer study demonstrates improved reproducibility in quantifying rodent pulmonary fibrosis with a modified Ashcroft scale, indicating a more reliable standard for histological...

Standardized Quantification of Pulmonary Fibrosis in Histological Samples

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Standardized Quantification of Pulmonary Fibrosis in Histological Samples

Key Points

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Implication

Cite This Study

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