What question did this study set out to answer?

To identify and characterize transcription factors that bind to the dehydration-responsive CATGTG cis-element within the ERD1 promoter of Arabidopsis thaliana.

September 1, 2004The Plant CellOpen Access

Isolation and Functional Analysis of Arabidopsis Stress-Inducible NAC Transcription Factors That Bind to a Drought-Responsivecis-Element in theearly responsive to dehydration stress 1PromoterW

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Authors

LTLam‐Son Phan TranTexas Tech University KNKazuo NakashimaJapan International Research Center for Agricultural Sciences YSYoh SakumaEhime University

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Implication

Functional genetic analysis reveals enhanced stress resistance and gene activation in Arabidopsis thaliana, suggesting critical roles for NAC transcription factors in drought response.

Key Points

To identify and characterize transcription factors that bind to the dehydration-responsive CATGTG cis-element within the ERD1 promoter of Arabidopsis thaliana.
Screened Arabidopsis cDNA libraries using a yeast one-hybrid system against a 63-bp erd1 promoter fragment containing the CATGTG motif.
Evaluated transactivation in Arabidopsis T87 protoplasts and localized tissue expression using histochemical GUS reporter constructs.
Performed microarray transcriptomic profiling and drought tolerance assays on transgenic Arabidopsis plants overexpressing ANAC019, ANAC055, or ANAC072.
Isolated three NAC transcription factors (ANAC019, ANAC055, ANAC072) that bound the CATGTG motif and were strongly induced by drought, salinity, and abscisic acid.
Defined the complete DNA recognition sequence for these NAC factors as CATGT harboring a CACG core binding site.
Overexpression of each NAC factor in transgenic plants upregulated multiple stress-responsive genes and significantly increased drought tolerance, though erd1 induction required additional factors.

Cite This Study

Tran et al. (2004) studied this question.

synapsesocial.com/papers/69d760feb843b2be9948f771 https://doi.org/10.1105/tpc.104.022699

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Implication

Functional genetic analysis reveals enhanced stress resistance and gene activation in Arabidopsis thaliana, suggesting critical roles for NAC transcription factors in drought response.

Isolation and Functional Analysis of Arabidopsis Stress-Inducible NAC Transcription Factors That Bind to a Drought-Responsivecis-Element in theearly responsive to dehydration stress 1PromoterW

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and Functional Analysis of Arabidopsis Stress-Inducible NAC Transcription Factors That Bind to a Drought-Responsivecis-Element in theearly responsive to dehydration stress 1PromoterW

Discussion

Member takes

Implication

Key Points

Cite This Study

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