What question did this study set out to answer?

Identify promoter fragments capable of driving transgene expression specifically in podocytes without non-specific activity in extrarenal tissues.

June 1, 2002Journal of the American Society of Nephrology

Two Gene Fragments that Direct Podocyte-Specific Expression in Transgenic Mice

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Authors

MMMarcus J. MoellerUniversitätsklinikum Aachen SSSilja K. SandenUniversity of Michigan ASAbdulsalam SoofiUniversity of Michigan

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Implication

Transgenic mouse study demonstrates podocyte-specific transgene expression using murine Nphs1 and human NPHS2 promoter fragments, highlighting a reliable tool for targeted kidney research.

Key Points

Identify promoter fragments capable of driving transgene expression specifically in podocytes without non-specific activity in extrarenal tissues.
Engineered transgenic mice carrying a 1.25-kb murine Nphs1 promoter fragment linked to a lacZ reporter gene (p1.25N-nlacF).
Engineered transgenic mice carrying a 2.5-kb human NPHS2 promoter fragment linked to a lacZ reporter gene (p2.5P-nlacF).
Evaluated beta-galactosidase reporter activity across adult tissues in founder lines and in mouse embryos from 8.5 days post coitus through birth.
The murine 1.25-kb Nphs1 promoter fragment drove beta-galactosidase expression in podocytes in 4 of 12 founder mice (33% penetrance), with no expression observed in the brain or pancreas.
The human 2.5-kb NPHS2 promoter fragment drove beta-galactosidase expression exclusively in podocytes in 12 of 12 founder lines (100% penetrance).
Neither construct exhibited reporter activity outside of the kidney in mouse embryos between embryonic day 8.5 and birth.

Cite This Study

Moeller et al. (2002) studied this question.

synapsesocial.com/papers/69d76362b1cb92dd1bb8ad95 https://doi.org/10.1097/01.asn.0000015614.68893.0b

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Implication

Transgenic mouse study demonstrates podocyte-specific transgene expression using murine Nphs1 and human NPHS2 promoter fragments, highlighting a reliable tool for targeted kidney research.

Two Gene Fragments that Direct Podocyte-Specific Expression in Transgenic Mice

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Implication

Key Points

Cite This Study

Two Gene Fragments that Direct Podocyte-Specific Expression in Transgenic Mice

Discussion

Member takes

Implication

Key Points

Cite This Study

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