What question did this study set out to answer?

Verify and improve taxonomic assignments across complete and draft prokaryotic genome assemblies in GenBank using average nucleotide identity (ANI).

May 24, 2018INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGYOpen Access

Using average nucleotide identity to improve taxonomic assignments in prokaryotic genomes at the NCBI

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Authors

SCStacy CiufoNational Institutes of Health SKSivakumar KannanNational Institutes of Health SSShobha SharmaVardhman Mahavir Medical College & Safdarjung Hospital

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Implication

Database analysis demonstrates improved taxonomic verification in prokaryotic GenBank assemblies using average nucleotide identity, indicating a reliable standard for microbial classification.

Key Points

Verify and improve taxonomic assignments across complete and draft prokaryotic genome assemblies in GenBank using average nucleotide identity (ANI).
Applied average nucleotide identity comparisons across all prokaryotic genome assemblies deposited in GenBank.
Established taxon-specific threshold ranges to systematically assess and verify taxonomic identities.
Integrated comparative algorithms into submission pipelines to confirm species designations for newly deposited genomes.
Reviewed and re-evaluated the taxonomic accuracy of existing complete and draft prokaryotic assemblies across GenBank.
Implemented a standardized, automated framework to confirm species assignments and flag misclassifications in incoming genome submissions.

Cite This Study

Ciufo et al. (2018) studied this question.

synapsesocial.com/papers/69d76bb3db9d5e1bf4b8a499 https://doi.org/10.1099/ijsem.0.002809

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Implication

Database analysis demonstrates improved taxonomic verification in prokaryotic GenBank assemblies using average nucleotide identity, indicating a reliable standard for microbial classification.

Using average nucleotide identity to improve taxonomic assignments in prokaryotic genomes at the NCBI

Discussion

Member takes

Implication

Key Points

Cite This Study

Using average nucleotide identity to improve taxonomic assignments in prokaryotic genomes at the NCBI

Discussion

Member takes

Implication

Key Points

Cite This Study

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