Methodological study demonstrates a colorimetric procedure for measuring transaminases in human serum, providing an accessible laboratory assay for clinical diagnosis.
Key Points
To develop a rapid, reliable colorimetric method for the routine clinical measurement of glutamic oxalacetic and glutamic pyruvic transaminases in blood serum.
Development of a chemical colorimetric technique to quantify serum glutamic oxalacetic transaminase and serum glutamic pyruvic transaminase activity.
Coupling of enzymatic transamination products with color reagents to measure optical density on standard laboratory spectrophotometers.
Permits straightforward photometric quantification of transaminase enzyme activity in human serum without requiring specialized ultraviolet spectrophotometry.
Yields consistent, reproducible measurements of serum transaminase levels suitable for routine diagnostic assessment of hepatic and cardiac conditions.