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January 9, 2015Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Cholesterol Loading Reprograms the MicroRNA-143/145–Myocardin Axis to Convert Aortic Smooth Muscle Cells to a Dysfunctional Macrophage-Like Phenotype

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Authors

YVYuliya VengrenyukHNHitoo NishiXLXiaochun Long

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Overview

Laboratory study demonstrates cholesterol-induced vascular smooth muscle cell transdifferentiation into macrophage-like cells, suggesting distinct contributions to plaque formation.

Key Points

  • To determine the molecular mechanism and functional consequences of cholesterol-induced phenotypic switching in vascular smooth muscle cells.
  • Subjected vascular smooth muscle cells (VSMCs) to cholesterol loading in vitro.
  • Evaluated the expression of the miR-143/145–myocardin axis alongside VSMC and macrophage-specific markers.
  • Compared transcriptomic signatures and functional characteristics of transdifferentiated cells to classical macrophages in human and mouse atherosclerotic plaques.
  • Cholesterol loading provoked VSMC phenotypic switching into a macrophage-like state by downregulating the miR-143/145–myocardin axis.
  • Transdifferentiated VSMCs stained positive for standard macrophage markers in human and mouse plaques but possessed transcriptional and functional profiles distinct from classical macrophages.

Cite This Study

Vengrenyuk et al. (2015) studied this question.

synapsesocial.com/papers/69d7c42005ee2ba81dbedc91https://doi.org/10.1161/atvbaha.114.304029
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