What question did this study set out to answer?

To develop a 3D-printable microplate system that maintains hydrogel geometry and automates expansion microscopy workflows without direct manual handling.

December 1, 2023Cell Reports Physical ScienceOpen Access

Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping

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Authors

RSRajpinder S. Seehra SWSamantha J. Warrington BABenjamin H.K. Allouis

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Overview

Experimental study demonstrates in situ expansion microscopy using 3D-printable microplates in human cells and fruit fly tissue, highlighting improved distortion mapping and workflow reproducibility.

Key Points

To develop a 3D-printable microplate system that maintains hydrogel geometry and automates expansion microscopy workflows without direct manual handling.
Engineered a 3D-printable microplate containing the entire expansion microscopy pipeline within individual wells for in situ imaging without manual hydrogel transfer.
Tracked isotropic gel expansion and mapped nanoscale spatial distortions by aligning pre- and post-expansion microscopy images.
Evaluated platform performance using single-color and multiplexed expansion microscopy across cultured HeLa cells and dissected pupal Drosophila melanogaster wing tissue.
Well-confined processing preserved hydrogel geometry and orientation, eliminating mechanical damage and enabling direct, reproducible registration of target regions.
High-fidelity distortion mapping accurately identified distortion-prone regions ranging from sub-cellular organelles to micron-scale tissue architectures.

Cite This Study

Seehra et al. (2023) studied this question.

synapsesocial.com/papers/69d7c9583b601d7be3ae2ecb https://doi.org/10.1016/j.xcrp.2023.101719

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December 1, 2023Cell Reports Physical ScienceOpen Access

Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping

View Full Paper

Ask AI

Bookmark

Authors

RSRajpinder S. Seehra SWSamantha J. Warrington BABenjamin H.K. Allouis

Discussion

Member takes

Overview

Experimental study demonstrates in situ expansion microscopy using 3D-printable microplates in human cells and fruit fly tissue, highlighting improved distortion mapping and workflow reproducibility.

Key Points

To develop a 3D-printable microplate system that maintains hydrogel geometry and automates expansion microscopy workflows without direct manual handling.
Engineered a 3D-printable microplate containing the entire expansion microscopy pipeline within individual wells for in situ imaging without manual hydrogel transfer.
Tracked isotropic gel expansion and mapped nanoscale spatial distortions by aligning pre- and post-expansion microscopy images.
Evaluated platform performance using single-color and multiplexed expansion microscopy across cultured HeLa cells and dissected pupal Drosophila melanogaster wing tissue.
Well-confined processing preserved hydrogel geometry and orientation, eliminating mechanical damage and enabling direct, reproducible registration of target regions.
High-fidelity distortion mapping accurately identified distortion-prone regions ranging from sub-cellular organelles to micron-scale tissue architectures.

Cite This Study

Seehra et al. (2023) studied this question.

synapsesocial.com/papers/69d7c9583b601d7be3ae2ecb https://doi.org/10.1016/j.xcrp.2023.101719

View Full Paper

Ask AI

Bookmark