What question did this study set out to answer?

To determine how the absence of the antiangiogenic matricellular protein thrombospondin-2 influences dermal wound repair, cell turnover, and extracellular matrix remodeling.

November 25, 2008Journal of Histochemistry & CytochemistryOpen Access

Enhanced Angiogenesis and Reduced Contraction in Thrombospondin-2–null Wounds Is Associated With Increased Levels of Matrix Metalloproteinases-2 and −9, and Soluble VEGF

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To determine how the absence of the antiangiogenic matricellular protein thrombospondin-2 influences dermal wound repair, cell turnover, and extracellular matrix remodeling.
Evaluated wound healing in wild-type and thrombospondin-2-null mice using incisional wounds tested by tensiometry.
Assessed full-thickness excisional wounds for cell apoptosis (TUNEL staining), proliferation (MIB-5 immunohistochemistry), and expression of MMP-2, MMP-9, TIMP-1, TIMP-2, and soluble VEGF.
Tested the functional contraction capacity of wound-derived fibroblasts in collagen gel assays.
Incisional wound tensile strength recovery, as well as apoptosis and proliferation rates in excisional wounds, were equivalent between wild-type and thrombospondin-2-null mice.
Thrombospondin-2-null wounds exhibited elevated levels of MMP-2, MMP-9, TIMP-1, TIMP-2, and soluble VEGF compared to controls.
Fibroblasts lacking thrombospondin-2 displayed impaired collagen gel contraction capacity despite maintaining normal myofibroblast counts in the wound tissue.

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Implication

Animal study reveals increased angiogenesis and impaired contraction in thrombospondin-2–null murine wounds, indicating a critical role in extracellular matrix remodeling.

Cite This Study

MacLauchlan et al. (2008) studied this question.

synapsesocial.com/papers/69d7ca1a7392c8ce61bedbee https://doi.org/10.1369/jhc.2008.952689

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Implication

Animal study reveals increased angiogenesis and impaired contraction in thrombospondin-2–null murine wounds, indicating a critical role in extracellular matrix remodeling.

Enhanced Angiogenesis and Reduced Contraction in Thrombospondin-2–null Wounds Is Associated With Increased Levels of Matrix Metalloproteinases-2 and −9, and Soluble VEGF

Key Points

Discussion

Implication

Cite This Study

Enhanced Angiogenesis and Reduced Contraction in Thrombospondin-2–null Wounds Is Associated With Increased Levels of Matrix Metalloproteinases-2 and −9, and Soluble VEGF

Key Points

Discussion

Implication

Cite This Study

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