What question did this study set out to answer?

To determine how chaperone-mediated negative feedback regulates the activity and stability of the heat shock transcription factor sigma32 in Escherichia coli.

October 29, 2007Proceedings of the National Academy of SciencesOpen Access

Analysis of σ ³² mutants defective in chaperone-mediated feedback control reveals unexpected complexity of the heat shock response

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To determine how chaperone-mediated negative feedback regulates the activity and stability of the heat shock transcription factor sigma32 in Escherichia coli.
Isolated and identified point mutations in sigma32 that impair chaperone-mediated feedback control.
Evaluated in vivo protein stability and resistance to inhibition by DnaK/J and GroEL/S chaperone systems.
Assayed in vitro binding affinity to chaperones and RNA polymerase, along with chaperone-mediated transcriptional inactivation.
Identified sigma32 mutants that are resistant to in vivo inhibition by both DnaK/J and GroEL/S chaperones while exhibiting markedly increased protein stability.
Demonstrated that the most defective mutant retains nearly normal binding to chaperones and RNA polymerase and remains competent for in vitro chaperone inactivation, indicating an inhibition step downstream of binding.

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Implication

Mutational analysis uncovers chaperone resistance and elevated stability of sigma32 in Escherichia coli, suggesting regulation occurs downstream of chaperone binding.

Cite This Study

Yura et al. (2007) studied this question.

synapsesocial.com/papers/69d7daf061e2ce1627d18404 https://doi.org/10.1073/pnas.0708819104

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Implication

Mutational analysis uncovers chaperone resistance and elevated stability of sigma32 in Escherichia coli, suggesting regulation occurs downstream of chaperone binding.

Analysis of σ ³² mutants defective in chaperone-mediated feedback control reveals unexpected complexity of the heat shock response

Key Points

Discussion

Implication

Cite This Study

Analysis of σ ³² mutants defective in chaperone-mediated feedback control reveals unexpected complexity of the heat shock response

Key Points

Discussion

Implication

Cite This Study

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