What question did this study set out to answer?

To develop an efficient Agrobacterium tumefaciens-mediated transformation system for rice (Oryza sativa L.) and examine the structural boundaries of integrated T-DNA.

August 1, 1994The Plant JournalOpen Access

Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T‐DNA

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Authors

YHYukoh HieiJapan Tobacco (United States)SOShozo OhtaNagoya University TKToshihiko KomariKaneka (United States)

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Implication

Experimental study demonstrates efficient Agrobacterium-mediated gene transfer in rice using super-binary vectors, indicating monocots achieve transformation rates typical of dicots.

Key Points

To develop an efficient Agrobacterium tumefaciens-mediated transformation system for rice (Oryza sativa L.) and examine the structural boundaries of integrated T-DNA.
Co-cultivated scutellum-derived calli from various japonica rice cultivars, including Koshihikari, with an Agrobacterium tumefaciens strain harboring a super-binary vector.
Assessed transgene integration, expression, and inheritance across R0, R1, and R2 generations using genetic and molecular assays.
Sequenced the T-DNA integration junctions in transformed plants to compare boundary structures with those observed in dicotyledonous hosts.
Yielded high numbers of morphologically normal, fertile transgenic rice plants with transformation efficiencies comparable to routine Agrobacterium-mediated dicot protocols.
Demonstrated stable integration, robust expression, and mendelian inheritance of transgenes across the R0, R1, and R2 generations.
Confirmed through sequence analysis that T-DNA boundaries in transgenic rice are essentially identical to those in dicots, with high transformation success even in recalcitrant cultivars like Koshihikari.

Cite This Study

Hiei et al. (1994) studied this question.

synapsesocial.com/papers/69d7dc0c5c3030ff03d17ea9 https://doi.org/10.1046/j.1365-313x.1994.6020271.x

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Implication

Experimental study demonstrates efficient Agrobacterium-mediated gene transfer in rice using super-binary vectors, indicating monocots achieve transformation rates typical of dicots.

Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T‐DNA

Discussion

Member takes

Implication

Key Points

Cite This Study

Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T‐DNA

Discussion

Member takes

Implication

Key Points

Cite This Study

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