What question did this study set out to answer?

Develop a high-affinity fluorogenic RNA aptamer system to enable real-time tracking, visualization, and purification of RNA complexes.

August 7, 2014ACS Chemical Biology

RNA Mango Aptamer-Fluorophore: A Bright, High-Affinity Complex for RNA Labeling and Tracking

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Authors

EDElena V. DolgosheinaSimon Fraser University SJSunny C.Y. JengSimon Fraser University SPS. PanchapakesanSimon Fraser University

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Implication

Experimental study demonstrates live-cell RNA tracking and purification using the RNA Mango aptamer system, highlighting its potential for visualizing cellular RNA dynamics.

Key Points

Develop a high-affinity fluorogenic RNA aptamer system to enable real-time tracking, visualization, and purification of RNA complexes.
Selected the RNA Mango aptamer to bind thiazole orange fluorophore derivatives with nanomolar affinity.
Evaluated RNA Mango imaging via single-molecule fluorescence microscopy and microinjection into Caenorhabditis elegans gonads.
Engineered RNA Mango into a stem-loop region of bacterial 6S RNA paired with a biotinylated fluorophore for simultaneous labeling and isolation.
RNA Mango bound thiazole orange derivatives with nanomolar affinity, increasing fluorophore fluorescence up to 1,100-fold.
Single-molecule microscopy and in vivo imaging confirmed effective visualization of the aptamer-fluorophore complex inside C. elegans gonads.
Insertion of the aptamer into bacterial 6S RNA enabled concurrent fluorescent labeling and biotin-mediated affinity purification of functional RNA.

Cite This Study

Dolgosheina et al. (2014) studied this question.

synapsesocial.com/papers/69d7ec4f319e71454dbedd7c https://doi.org/10.1021/cb500499x

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Implication

Experimental study demonstrates live-cell RNA tracking and purification using the RNA Mango aptamer system, highlighting its potential for visualizing cellular RNA dynamics.

RNA Mango Aptamer-Fluorophore: A Bright, High-Affinity Complex for RNA Labeling and Tracking

Discussion

Member takes

Implication

Key Points

Cite This Study

RNA Mango Aptamer-Fluorophore: A Bright, High-Affinity Complex for RNA Labeling and Tracking

Discussion

Member takes

Implication

Key Points

Cite This Study

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