Why the study?
PCV3 poses a serious threat to the pig industry with no commercially available vaccine, making PCV3 capsid protein an ideal candidate for serodiagnosis.
Does the novel fully automated CLEIA accurately and efficiently detect antibodies against PCV3 Cap in porcine serum compared to a commercially available kit?
Does the novel fully automated CLEIA accurately and efficiently detect antibodies against PCV3 Cap in porcine serum compared to a commercially available kit?
The novel fully automated CLEIA provides a rapid, reliable, and labor-saving tool for the serodiagnosis and epidemiological surveillance of PCV3.
Answers are grounded in this paper and cite their sources.
Offers reliable automated PCV3 serodiagnosis in swine; leaves open field validation before routine use.
Porcine circovirus 3 (PCV3) is a small non-enveloped circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS). It has occurred worldwide and poses a serious threat to the pig industry. However, there is no commercially available vaccine. PCV3 capsid protein (Cap) is an ideal antigen candidate for serodiagnosis. Here, a novel fully automated chemiluminescence enzyme immunoassay (CLEIA) was developed to detect antibodies (Abs) to Cap in porcine serum. Recombinant PCV3 Cap, self-assembled into virus-like particles (VLPs), was produced using baculovirus and coupled to magnetic particles (Cap-MPs) as carriers. Combined with an alkaline phosphatase (AP)-adamantane (AMPPD) system, Cap-Abs can be rapidly measured on a fully automated chemiluminescence analyzer. Under optimal conditions, a cut-off value of 31,508 was determined, with a diagnostic sensitivity of 96.8% and specificity of 97.3%. No cross-reactivity was observed with PCV1 and PCV2 and other common porcine pathogens, and both intra-assay and inter-assay coefficients were less than 5% and 10%, respectively. Prepared Cap-MPs can be stored at 4 °C for more than 6 months. Importantly, this CLEIA had a good agreement of 95.19% with the commercially available kit, demonstrating excellent analytical sensitivity and significantly reduced operating time and labor. A serological survey was then conducted, and showed that PCV3 continues to spread widely in South China. In conclusion, our CLEIA provides time and labor-saving, and a reliable tool for PCV3 epidemiological surveillance.
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Wang et al. (2024) studied this question.
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