Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism

Lignin sources affect enzyme adsorption using structural features and lignin composition, such as S/G ratio, carboxylic acid, aliphatic hydroxyl, and phenolic hydroxyl. For mono-component enzymes, the adsorption capacity decreased in the order CBH, xylanase > EG > BG. These investigations revealed the difference in lignin properties between diverse biomass and adsorption capacity of enzymes to lignins, and the possible underlying mechanism. The results can also serve as a reference for the genetic modification of lignocellulosic biomass and enzymes.