What question did this study set out to answer?

To determine whether an electric field can drive individual single-stranded RNA and DNA molecules through a nanoscale membrane channel for single-molecule characterization.

November 26, 1996Proceedings of the National Academy of SciencesOpen Access

Characterization of individual polynucleotide molecules using a membrane channel

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Authors

JKJohn J. KasianowiczUniversity of Freiburg EBEric BrandinBoston University DBDaniel BrantonBoston University

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Implication

Biophysical experiment demonstrates electric-field-driven translocation of single polynucleotides through a membrane channel, indicating potential for high-speed single-molecule sequencing.

Key Points

To determine whether an electric field can drive individual single-stranded RNA and DNA molecules through a nanoscale membrane channel for single-molecule characterization.
Applied an electric field across a lipid bilayer membrane containing a 2.6-nm diameter ion channel.
Measured ionic current blockades during the electrophoretic translocation of single-stranded DNA and RNA molecules.
Single-stranded DNA and RNA molecules traverse the 2.6-nm channel as extended chains, causing transient decreases in ionic current.
The duration of the ionic current blockade is directly proportional to polynucleotide length, enabling polymer length determination.

Cite This Study

Kasianowicz et al. (1996) studied this question.

synapsesocial.com/papers/69d84edd05ee2ba81dbef871 https://doi.org/10.1073/pnas.93.24.13770

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Implication

Biophysical experiment demonstrates electric-field-driven translocation of single polynucleotides through a membrane channel, indicating potential for high-speed single-molecule sequencing.

Characterization of individual polynucleotide molecules using a membrane channel

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of individual polynucleotide molecules using a membrane channel

Discussion

Member takes

Implication

Key Points

Cite This Study

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