What question did this study set out to answer?

To establish an in vitro culture system for expanding undifferentiated human mammary stem and progenitor cells and characterize their transcriptional profiles and lineage differentiation potential.

May 15, 2003Genes & DevelopmentOpen Access

In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells

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Authors

GDGabriela DontuUniversity of Michigan WAWissam AbdallahNorthwell Health JFJessica FoleyTufts University

Discussion

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Implication

In vitro study demonstrates propagation and multilineage differentiation of human mammary stem cells as mammospheres, highlighting regulatory pathways in development and carcinogenesis.

Key Points

To establish an in vitro culture system for expanding undifferentiated human mammary stem and progenitor cells and characterize their transcriptional profiles and lineage differentiation potential.
Cultured human mammary epithelial cells (HMECs) in nonadherent suspension conditions to promote the formation of mammospheres.
Evaluated stem cell self-renewal and lineage potency by inducing differentiation along mammary epithelial lineages in reconstituted 3D culture systems.
Performed gene expression profiling on cells isolated from mammospheres to identify stem cell-associated genetic programs and novel candidate biomarkers.
Suspension culture as nonadherent mammospheres successfully maintained HMECs in an undifferentiated state enriched for functional progenitor and stem cells.
Mammosphere-derived cells clonally generated complex, functional ductal-alveolar structures and differentiated into all three mammary epithelial lineages in 3D culture.
Transcriptional profiling demonstrated shared molecular programs with other stem/progenitor cell types and revealed specific gene markers characteristic of mammary stem cells.

Cite This Study

Dontu et al. (2003) studied this question.

synapsesocial.com/papers/69d85580c025a7c015beda33 https://doi.org/10.1101/gad.1061803

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May 15, 2003Genes & DevelopmentOpen Access

In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells

View Full Paper

Ask AI

Bookmark

Authors

GDGabriela DontuUniversity of Michigan WAWissam AbdallahNorthwell Health JFJessica FoleyTufts University

Discussion

Member takes

Implication

In vitro study demonstrates propagation and multilineage differentiation of human mammary stem cells as mammospheres, highlighting regulatory pathways in development and carcinogenesis.

Key Points

To establish an in vitro culture system for expanding undifferentiated human mammary stem and progenitor cells and characterize their transcriptional profiles and lineage differentiation potential.
Cultured human mammary epithelial cells (HMECs) in nonadherent suspension conditions to promote the formation of mammospheres.
Evaluated stem cell self-renewal and lineage potency by inducing differentiation along mammary epithelial lineages in reconstituted 3D culture systems.
Performed gene expression profiling on cells isolated from mammospheres to identify stem cell-associated genetic programs and novel candidate biomarkers.
Suspension culture as nonadherent mammospheres successfully maintained HMECs in an undifferentiated state enriched for functional progenitor and stem cells.
Mammosphere-derived cells clonally generated complex, functional ductal-alveolar structures and differentiated into all three mammary epithelial lineages in 3D culture.
Transcriptional profiling demonstrated shared molecular programs with other stem/progenitor cell types and revealed specific gene markers characteristic of mammary stem cells.

Cite This Study

Dontu et al. (2003) studied this question.

synapsesocial.com/papers/69d85580c025a7c015beda33 https://doi.org/10.1101/gad.1061803

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