January 1, 2005Genes & DevelopmentOpen Access

Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms

View Full Paper

Ask AI

Bookmark

Population

Mammalian cell extracts

Comparison

Depletion of poly-binding protein and… vs Unsupplemented PABP-depleted extracts

Design

Preclinical

Authors

AKAvak KahvejianFlagship Pioneering (United States)YSYuri V. SvitkinMcGill University RSRami SukariehAgency for Science, Technology and Research

Discussion

Member takes

Implication

Hypothesis-generating in animal extracts; leaves open any clinical relevance or therapeutic targeting of PABP.

Structured PICO

Population

Mammalian cell extracts

Intervention

Depletion of poly(A)-binding protein (PABP) and supplementation with wild-type or mutant (M161A) PABP

Comparator

Unsupplemented PABP-depleted extracts

Outcome

Rates of translation and efficiency of 48S and 80S ribosome initiation complex formationsurrogate

PABP functions as a canonical translation initiation factor, playing a direct role in 60S subunit joining and initiation complex formation at the 5'-end of mRNA.

Cite This Study

Kahvejian et al. (2005) studied this question.

synapsesocial.com/papers/69d88f2fc025a7c015bee153 https://doi.org/10.1101/gad.1262905

View Full Paper

Ask AI

Bookmark

Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider