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April 10, 2026Small

High‐Throughput Single‐Cell Biochip System for Functional Interrogation of Protein Interactions in Living Cells

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Authors

FLFeng LiuCWChengbao WuCYChenying Yang

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Overview

This platform detects protein interactions in living cells, suggesting new insights into cellular behaviors.

Key Points

  • The aim is to investigate protein-protein interactions in live cells with single-cell resolution to understand regulatory mechanisms.
  • Developed a biochip system named 'Pixar' for PPI detection
  • Utilized focused electric field-based delivery for peptide-tagged protein plasmids
  • Implemented a high-throughput single-cell capture array for dynamic profiling
  • Achieved over 90% delivery efficiency and cell viability
  • Enabled monitoring of tumor-associated AKT-mTOR interactions
  • Facilitated analysis of heterogeneous cell migration and proliferation across thousands of cells

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/69d893a86c1944d70ce0499chttps://doi.org/10.1002/smll.73336
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