In vivo and in vitro analysis evaluates quebracho-chestnut tannin extract's effects on nematodes in lambs, suggesting limitations in practical applications.
Key Points
This work aims to evaluate the anthelmintic activity of quebracho-chestnut tannin extract against Haemonchus contortus in lambs both in vivo and in vitro.
Conducted in vitro assays for egg hatching and larval migration inhibition to find effective concentrations.
Performed an in vivo trial with 18 lambs divided into three groups receiving varied tannin doses.
Analyzed faecal egg counts, larval counts from coprocultures, and adult worm recovery post-treatment.
In vitro, tannin extract inhibited egg hatching and larval migration at low concentrations (3.8 mg/mL and 1.4 mg/mL).
No significant effects were observed in faecal egg counts or adult worm recovery in vivo at both 0.3% and 3% doses.
Scanning electron microscopy revealed significant damage to the cuticle of adult worms and eggs, indicating potential reproductive interference.