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Classify cellular diversity and regulatory mechanisms in the mouse cerebral cortex and hippocampus using single-cell transcriptomics.

February 21, 2015ScienceOpen Access

Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

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Classify cellular diversity and regulatory mechanisms in the mouse cerebral cortex and hippocampus using single-cell transcriptomics.
Performed large-scale single-cell RNA sequencing (RNA-seq) on cells isolated from the mouse somatosensory cortex and hippocampal CA1 region.
Identified marker genes across cell populations to align transcriptional profiles with known cellular morphology, spatial distribution, and developmental lineages.
Identified 47 molecularly distinct subclasses covering all major cortical cell types, including neurons, glia, and vascular cells.
Characterized distinct novel cell subpopulations, including a Pax6-expressing layer I interneuron and an Itpr2-positive postmitotic oligodendrocyte subclass.
Determined that combinations of transcription factors establish a layered regulatory code associated with preserving adult cell identity.

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Single-cell transcriptomic profiling uncovers 47 distinct cell subclasses in mouse cortex and hippocampus, suggesting a layered transcriptional regulatory code maintains cell identity.

Cite This Study

Zeisel et al. (2015) studied this question.

synapsesocial.com/papers/69d898c918b0ca7f91d185fc https://doi.org/10.1126/science.aaa1934

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Implication

Single-cell transcriptomic profiling uncovers 47 distinct cell subclasses in mouse cortex and hippocampus, suggesting a layered transcriptional regulatory code maintains cell identity.

Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

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Discussion

Implication

Cite This Study

Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

Key Points

Discussion

Implication

Cite This Study

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