What question did this study set out to answer?

To establish a rapid, robust mass-spectrometry-based pipeline for high-throughput, quantitative plasma proteome profiling from minimal blood volumes without requiring protein depletion.

March 1, 2016Cell SystemsOpen Access

Plasma Proteome Profiling to Assess Human Health and Disease

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To establish a rapid, robust mass-spectrometry-based pipeline for high-throughput, quantitative plasma proteome profiling from minimal blood volumes without requiring protein depletion.
Developed a single-run shotgun proteomic workflow analyzing 1 μl plasma samples from single finger pricks across 20-minute analytical gradients.
Implemented a label-free quantification strategy without sample protein depletion.
Utilized simple peptide pre-fractionation to evaluate a deeper, 1,000-protein plasma proteome.
Reproducibly quantified the apolipoprotein family, sex-related proteins, C-reactive protein, and >40 FDA-approved biomarkers with a coefficient of variation <20% using label-free quantification.
Achieved deep functional profiling of 1,000 plasma proteins via peptide pre-fractionation.

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Implication

Methodological study demonstrates rapid plasma proteome profiling from finger-prick blood samples, indicating potential for large-scale biomedical monitoring.

Cite This Study

Geyer et al. (2016) studied this question.

synapsesocial.com/papers/69d8997752654bb436d1975d https://doi.org/10.1016/j.cels.2016.02.015

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Implication

Methodological study demonstrates rapid plasma proteome profiling from finger-prick blood samples, indicating potential for large-scale biomedical monitoring.

Plasma Proteome Profiling to Assess Human Health and Disease

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Discussion

Implication

Cite This Study

Plasma Proteome Profiling to Assess Human Health and Disease

Key Points

Discussion

Implication

Cite This Study

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