Stability of DNA Origami Nanostructure under Diverse Chemical Environments

We report the effect of chemical and physical treatments on the structural stability of DNA origami nanostructures. Our result shows that DNA nanostructure maintains its shape under harsh processing conditions, including thermal annealing up to 200 °C for 10 min, immersing in a wide range of organic solvents for up to 24 h, brief exposure to alkaline aqueous solutions, and 5 min exposure to UV/O3. Our result suggests that the application window of DNA nanostructure is significantly wider than previously believed.