What question did this study set out to answer?

To determine the role of G proteins in coupling plasma membrane-associated estrogen receptor alpha to the rapid activation of endothelial nitric-oxide synthase.

July 1, 2001Journal of Biological ChemistryOpen Access

Plasma Membrane Estrogen Receptors Are Coupled to Endothelial Nitric-oxide Synthase through Gαi

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Authors

MWMyra H. WyckoffUniversity of Iowa KCKen L. ChamblissThe University of Texas Southwestern Medical Center CMChieko MineoThe University of Texas Southwestern Medical Center

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Implication

In vitro study demonstrates that estrogen activates endothelial nitric oxide synthase through plasma membrane receptor coupling to Gai, indicating a cell-surface steroid signaling pathway.

Key Points

To determine the role of G proteins in coupling plasma membrane-associated estrogen receptor alpha to the rapid activation of endothelial nitric-oxide synthase.
Measured nitric oxide synthase activity in intact endothelial cells, isolated endothelial plasma membranes, and transfected COS-7 cells exposed to estradiol-17β, acetylcholine, pertussis toxin, or GDPβS.
Performed coimmunoprecipitation on plasma membranes from endothelial cells and COS-7 cells co-transfected with ERα and specific Gα proteins (Gαi, Gαq, or Gαs) in the presence or absence of ICI 182,780 and pertussis toxin.
Quantified estradiol-mediated eNOS activation in COS-7 cells following cotransfection with Gαi or the G-protein signaling regulator RGS4.
Estradiol-17β (10⁻⁸ M) increased nitric oxide synthase activity within 15 minutes to a degree comparable to acetylcholine, an effect completely inhibited by pertussis toxin and GDPβS.
Estradiol stimulated direct physical binding between ERα and Gαi in endothelial and COS-7 membranes, which was blocked by ICI 182,780 and pertussis toxin, whereas no binding occurred with Gαq or Gαs.
Cotransfection of Gαi into ERα- and eNOS-expressing COS-7 cells produced a 3-fold increase in estradiol-mediated eNOS activation, whereas cotransfection of RGS4 inhibited this stimulation.

Cite This Study

Wyckoff et al. (2001) studied this question.

synapsesocial.com/papers/69d8beb5ce048d2571bedff1 https://doi.org/10.1074/jbc.m100312200

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Implication

In vitro study demonstrates that estrogen activates endothelial nitric oxide synthase through plasma membrane receptor coupling to Gai, indicating a cell-surface steroid signaling pathway.

Plasma Membrane Estrogen Receptors Are Coupled to Endothelial Nitric-oxide Synthase through Gαi

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Key Points

Cite This Study

Plasma Membrane Estrogen Receptors Are Coupled to Endothelial Nitric-oxide Synthase through Gαi

Discussion

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Implication

Key Points

Cite This Study

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