What question did this study set out to answer?

To review the general procedures, practical pitfalls, troubleshooting steps, and interpretation methods of immunohistochemistry for practicing pathologists and pathology trainees.

November 4, 2016Journal of Pathology and Translational MedicineOpen Access

Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips

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Authors

SKSo‐Woon KimKyung Hee University JRJin RohAjou University CPChan-Sik ParkAsan Medical Center

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Implication

Narrative review details standard protocols, troubleshooting steps, and interpretation strategies in pathology, highlighting essential methods to minimize diagnostic errors.

Key Points

To review the general procedures, practical pitfalls, troubleshooting steps, and interpretation methods of immunohistochemistry for practicing pathologists and pathology trainees.
Reviewed standard step-by-step procedures for immunohistochemistry across pre-analytical, analytical, and post-analytical stages.
Synthesized technical troubleshooting strategies alongside general scoring and quantitative analysis methods.
Identified critical protocol optimization steps required at each phase of staining to mitigate false-positive and false-negative artifacts.
Outlined systematic approaches for accurate slide interpretation and marker quantification within complex tissue microenvironments.

Cite This Study

Kim et al. (2016) studied this question.

synapsesocial.com/papers/69d8d424183921ebcaae3d2f https://doi.org/10.4132/jptm.2016.08.08

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Implication

Narrative review details standard protocols, troubleshooting steps, and interpretation strategies in pathology, highlighting essential methods to minimize diagnostic errors.

Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips

Discussion

Member takes

Implication

Key Points

Cite This Study

Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips

Discussion

Member takes

Implication

Key Points

Cite This Study

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